Review



eppendorf n a thermal mixer  (Eppendorf AG)


Bioz Verified Symbol Eppendorf AG is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Eppendorf AG eppendorf n a thermal mixer
    Eppendorf N A Thermal Mixer, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 204617 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thermal+mixer/Eppendorf/pm42784455-98-156-156
    Average 99 stars, based on 204617 article reviews
    eppendorf n a thermal mixer - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Protein expression plasmid vector of your choice containing a blunt-end restriction site at the multi-cloning site (see protocol introduction) Midiprep Plasmid DNA kit (Qiagen Plasmid Midi Kit 100]; Qiagen, cat. no. 12145) Nuclease free water (NF-water, NEB, cat. no B1500) Blunt-end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S) Sterile LB liquid medium (Thermo Fisher, cat. no. L3522-1KG) 10× CutSmart Buffer (NEB, cat. no B6004) Alkaline phosphatase (Quick CIP or rSAP, NEB, cat. no. M0525S or M0371S) 0.7% fresh agarose gel (SeaKem LE Agarose; Lonza, cat. no. 50004) AccuGENE 5× TBE Buffer (Lonza, cat. no. 50836) Monarch DNA Gel Extraction Kit (NEB, cat. no. T1020S) 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standards #1 and #2 (Invitrogen, cat. no. Q33263) Gel box and gel electrophoresis apparatus for agarose gel Razor blade (VWR, cat. no. 55411-050) Thermal mixer (Eppendorf, cat. no. 5382000023) 1.5-ml tube (Eppendorf, cat. no. 022363301) Qubit 4 Fluorometer (Invitrogen, cat. no. Q33238) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Florez-Cardona et al. 18 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Table 5 Reaction Components for Digesting Expression Plasmid to Create Linearized Vector Component Volume Nuclease-free water a Vector a 10× CutSmart buffer 5 μl SmaI 4 μl Total volume 50 μl a Nuclease-free water, vector, and CutSmart buffer are from the midiprep kit.

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Protein expression plasmid vector of your choice containing a blunt‐end restriction site at the multi‐cloning site (see protocol introduction) Midiprep Plasmid DNA kit (Qiagen Plasmid Midi Kit 100]; Qiagen, cat. no. 12145) Nuclease free water (NF‐water, NEB, cat. no B1500) Blunt‐end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S) Sterile LB liquid medium (Thermo Fisher, cat. no. L3522‐1KG) 10× CutSmart Buffer (NEB, cat. no B6004) Alkaline phosphatase (Quick CIP or rSAP, NEB, cat. no. M0525S or M0371S) 0.7% fresh agarose gel (SeaKem LE Agarose; Lonza, cat. no. 50004) AccuGENE 5× TBE Buffer (Lonza, cat. no. 50836) Monarch DNA Gel Extraction Kit (NEB, cat. no. T1020S) 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standards #1 and #2 (Invitrogen, cat. no. Q33263) Gel box and gel electrophoresis apparatus for agarose gel Razor blade (VWR, cat. no. 55411‐050) Thermal mixer (Eppendorf, cat. no. 5382000023) 1.5‐ml tube (Eppendorf, cat. no. 022363301) Qubit 4 Fluorometer (Invitrogen, cat. no. Q33238) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) .. 125 g sucrose (Sigma‐Aldrich, cat. no. S0389‐500G) 25 ml 1 M Tris•Cl, pH 8.0 (Invitrogen, cat. no. AM9855G) Add deionized H 2 O to 500 ml final volume Store up to 6 months at 4°C.

    Plasmid Preparation:

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Protein expression plasmid vector of your choice containing a blunt-end restriction site at the multi-cloning site (see protocol introduction) Midiprep Plasmid DNA kit (Qiagen Plasmid Midi Kit 100]; Qiagen, cat. no. 12145) Nuclease free water (NF-water, NEB, cat. no B1500) Blunt-end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S) Sterile LB liquid medium (Thermo Fisher, cat. no. L3522-1KG) 10× CutSmart Buffer (NEB, cat. no B6004) Alkaline phosphatase (Quick CIP or rSAP, NEB, cat. no. M0525S or M0371S) 0.7% fresh agarose gel (SeaKem LE Agarose; Lonza, cat. no. 50004) AccuGENE 5× TBE Buffer (Lonza, cat. no. 50836) Monarch DNA Gel Extraction Kit (NEB, cat. no. T1020S) 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standards #1 and #2 (Invitrogen, cat. no. Q33263) Gel box and gel electrophoresis apparatus for agarose gel Razor blade (VWR, cat. no. 55411-050) Thermal mixer (Eppendorf, cat. no. 5382000023) 1.5-ml tube (Eppendorf, cat. no. 022363301) Qubit 4 Fluorometer (Invitrogen, cat. no. Q33238) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Florez-Cardona et al. 18 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Table 5 Reaction Components for Digesting Expression Plasmid to Create Linearized Vector Component Volume Nuclease-free water a Vector a 10× CutSmart buffer 5 μl SmaI 4 μl Total volume 50 μl a Nuclease-free water, vector, and CutSmart buffer are from the midiprep kit.

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Protein expression plasmid vector of your choice containing a blunt‐end restriction site at the multi‐cloning site (see protocol introduction) Midiprep Plasmid DNA kit (Qiagen Plasmid Midi Kit 100]; Qiagen, cat. no. 12145) Nuclease free water (NF‐water, NEB, cat. no B1500) Blunt‐end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S) Sterile LB liquid medium (Thermo Fisher, cat. no. L3522‐1KG) 10× CutSmart Buffer (NEB, cat. no B6004) Alkaline phosphatase (Quick CIP or rSAP, NEB, cat. no. M0525S or M0371S) 0.7% fresh agarose gel (SeaKem LE Agarose; Lonza, cat. no. 50004) AccuGENE 5× TBE Buffer (Lonza, cat. no. 50836) Monarch DNA Gel Extraction Kit (NEB, cat. no. T1020S) 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standards #1 and #2 (Invitrogen, cat. no. Q33263) Gel box and gel electrophoresis apparatus for agarose gel Razor blade (VWR, cat. no. 55411‐050) Thermal mixer (Eppendorf, cat. no. 5382000023) 1.5‐ml tube (Eppendorf, cat. no. 022363301) Qubit 4 Fluorometer (Invitrogen, cat. no. Q33238) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) .. 125 g sucrose (Sigma‐Aldrich, cat. no. S0389‐500G) 25 ml 1 M Tris•Cl, pH 8.0 (Invitrogen, cat. no. AM9855G) Add deionized H 2 O to 500 ml final volume Store up to 6 months at 4°C.

    Sterility:

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Protein expression plasmid vector of your choice containing a blunt-end restriction site at the multi-cloning site (see protocol introduction) Midiprep Plasmid DNA kit (Qiagen Plasmid Midi Kit 100]; Qiagen, cat. no. 12145) Nuclease free water (NF-water, NEB, cat. no B1500) Blunt-end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S) Sterile LB liquid medium (Thermo Fisher, cat. no. L3522-1KG) 10× CutSmart Buffer (NEB, cat. no B6004) Alkaline phosphatase (Quick CIP or rSAP, NEB, cat. no. M0525S or M0371S) 0.7% fresh agarose gel (SeaKem LE Agarose; Lonza, cat. no. 50004) AccuGENE 5× TBE Buffer (Lonza, cat. no. 50836) Monarch DNA Gel Extraction Kit (NEB, cat. no. T1020S) 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standards #1 and #2 (Invitrogen, cat. no. Q33263) Gel box and gel electrophoresis apparatus for agarose gel Razor blade (VWR, cat. no. 55411-050) Thermal mixer (Eppendorf, cat. no. 5382000023) 1.5-ml tube (Eppendorf, cat. no. 022363301) Qubit 4 Fluorometer (Invitrogen, cat. no. Q33238) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Florez-Cardona et al. 18 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Table 5 Reaction Components for Digesting Expression Plasmid to Create Linearized Vector Component Volume Nuclease-free water a Vector a 10× CutSmart buffer 5 μl SmaI 4 μl Total volume 50 μl a Nuclease-free water, vector, and CutSmart buffer are from the midiprep kit.

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Protein expression plasmid vector of your choice containing a blunt‐end restriction site at the multi‐cloning site (see protocol introduction) Midiprep Plasmid DNA kit (Qiagen Plasmid Midi Kit 100]; Qiagen, cat. no. 12145) Nuclease free water (NF‐water, NEB, cat. no B1500) Blunt‐end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S) Sterile LB liquid medium (Thermo Fisher, cat. no. L3522‐1KG) 10× CutSmart Buffer (NEB, cat. no B6004) Alkaline phosphatase (Quick CIP or rSAP, NEB, cat. no. M0525S or M0371S) 0.7% fresh agarose gel (SeaKem LE Agarose; Lonza, cat. no. 50004) AccuGENE 5× TBE Buffer (Lonza, cat. no. 50836) Monarch DNA Gel Extraction Kit (NEB, cat. no. T1020S) 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standards #1 and #2 (Invitrogen, cat. no. Q33263) Gel box and gel electrophoresis apparatus for agarose gel Razor blade (VWR, cat. no. 55411‐050) Thermal mixer (Eppendorf, cat. no. 5382000023) 1.5‐ml tube (Eppendorf, cat. no. 022363301) Qubit 4 Fluorometer (Invitrogen, cat. no. Q33238) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) .. 125 g sucrose (Sigma‐Aldrich, cat. no. S0389‐500G) 25 ml 1 M Tris•Cl, pH 8.0 (Invitrogen, cat. no. AM9855G) Add deionized H 2 O to 500 ml final volume Store up to 6 months at 4°C.

    Agarose Gel Electrophoresis:

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Protein expression plasmid vector of your choice containing a blunt-end restriction site at the multi-cloning site (see protocol introduction) Midiprep Plasmid DNA kit (Qiagen Plasmid Midi Kit 100]; Qiagen, cat. no. 12145) Nuclease free water (NF-water, NEB, cat. no B1500) Blunt-end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S) Sterile LB liquid medium (Thermo Fisher, cat. no. L3522-1KG) 10× CutSmart Buffer (NEB, cat. no B6004) Alkaline phosphatase (Quick CIP or rSAP, NEB, cat. no. M0525S or M0371S) 0.7% fresh agarose gel (SeaKem LE Agarose; Lonza, cat. no. 50004) AccuGENE 5× TBE Buffer (Lonza, cat. no. 50836) Monarch DNA Gel Extraction Kit (NEB, cat. no. T1020S) 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standards #1 and #2 (Invitrogen, cat. no. Q33263) Gel box and gel electrophoresis apparatus for agarose gel Razor blade (VWR, cat. no. 55411-050) Thermal mixer (Eppendorf, cat. no. 5382000023) 1.5-ml tube (Eppendorf, cat. no. 022363301) Qubit 4 Fluorometer (Invitrogen, cat. no. Q33238) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Florez-Cardona et al. 18 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Table 5 Reaction Components for Digesting Expression Plasmid to Create Linearized Vector Component Volume Nuclease-free water a Vector a 10× CutSmart buffer 5 μl SmaI 4 μl Total volume 50 μl a Nuclease-free water, vector, and CutSmart buffer are from the midiprep kit.

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Protein expression plasmid vector of your choice containing a blunt‐end restriction site at the multi‐cloning site (see protocol introduction) Midiprep Plasmid DNA kit (Qiagen Plasmid Midi Kit 100]; Qiagen, cat. no. 12145) Nuclease free water (NF‐water, NEB, cat. no B1500) Blunt‐end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S) Sterile LB liquid medium (Thermo Fisher, cat. no. L3522‐1KG) 10× CutSmart Buffer (NEB, cat. no B6004) Alkaline phosphatase (Quick CIP or rSAP, NEB, cat. no. M0525S or M0371S) 0.7% fresh agarose gel (SeaKem LE Agarose; Lonza, cat. no. 50004) AccuGENE 5× TBE Buffer (Lonza, cat. no. 50836) Monarch DNA Gel Extraction Kit (NEB, cat. no. T1020S) 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standards #1 and #2 (Invitrogen, cat. no. Q33263) Gel box and gel electrophoresis apparatus for agarose gel Razor blade (VWR, cat. no. 55411‐050) Thermal mixer (Eppendorf, cat. no. 5382000023) 1.5‐ml tube (Eppendorf, cat. no. 022363301) Qubit 4 Fluorometer (Invitrogen, cat. no. Q33238) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) .. 125 g sucrose (Sigma‐Aldrich, cat. no. S0389‐500G) 25 ml 1 M Tris•Cl, pH 8.0 (Invitrogen, cat. no. AM9855G) Add deionized H 2 O to 500 ml final volume Store up to 6 months at 4°C.

    Gel Extraction:

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Protein expression plasmid vector of your choice containing a blunt-end restriction site at the multi-cloning site (see protocol introduction) Midiprep Plasmid DNA kit (Qiagen Plasmid Midi Kit 100]; Qiagen, cat. no. 12145) Nuclease free water (NF-water, NEB, cat. no B1500) Blunt-end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S) Sterile LB liquid medium (Thermo Fisher, cat. no. L3522-1KG) 10× CutSmart Buffer (NEB, cat. no B6004) Alkaline phosphatase (Quick CIP or rSAP, NEB, cat. no. M0525S or M0371S) 0.7% fresh agarose gel (SeaKem LE Agarose; Lonza, cat. no. 50004) AccuGENE 5× TBE Buffer (Lonza, cat. no. 50836) Monarch DNA Gel Extraction Kit (NEB, cat. no. T1020S) 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standards #1 and #2 (Invitrogen, cat. no. Q33263) Gel box and gel electrophoresis apparatus for agarose gel Razor blade (VWR, cat. no. 55411-050) Thermal mixer (Eppendorf, cat. no. 5382000023) 1.5-ml tube (Eppendorf, cat. no. 022363301) Qubit 4 Fluorometer (Invitrogen, cat. no. Q33238) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Florez-Cardona et al. 18 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Table 5 Reaction Components for Digesting Expression Plasmid to Create Linearized Vector Component Volume Nuclease-free water a Vector a 10× CutSmart buffer 5 μl SmaI 4 μl Total volume 50 μl a Nuclease-free water, vector, and CutSmart buffer are from the midiprep kit.

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Protein expression plasmid vector of your choice containing a blunt‐end restriction site at the multi‐cloning site (see protocol introduction) Midiprep Plasmid DNA kit (Qiagen Plasmid Midi Kit 100]; Qiagen, cat. no. 12145) Nuclease free water (NF‐water, NEB, cat. no B1500) Blunt‐end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S) Sterile LB liquid medium (Thermo Fisher, cat. no. L3522‐1KG) 10× CutSmart Buffer (NEB, cat. no B6004) Alkaline phosphatase (Quick CIP or rSAP, NEB, cat. no. M0525S or M0371S) 0.7% fresh agarose gel (SeaKem LE Agarose; Lonza, cat. no. 50004) AccuGENE 5× TBE Buffer (Lonza, cat. no. 50836) Monarch DNA Gel Extraction Kit (NEB, cat. no. T1020S) 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standards #1 and #2 (Invitrogen, cat. no. Q33263) Gel box and gel electrophoresis apparatus for agarose gel Razor blade (VWR, cat. no. 55411‐050) Thermal mixer (Eppendorf, cat. no. 5382000023) 1.5‐ml tube (Eppendorf, cat. no. 022363301) Qubit 4 Fluorometer (Invitrogen, cat. no. Q33238) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) .. 125 g sucrose (Sigma‐Aldrich, cat. no. S0389‐500G) 25 ml 1 M Tris•Cl, pH 8.0 (Invitrogen, cat. no. AM9855G) Add deionized H 2 O to 500 ml final volume Store up to 6 months at 4°C.

    Nucleic Acid Electrophoresis:

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Protein expression plasmid vector of your choice containing a blunt-end restriction site at the multi-cloning site (see protocol introduction) Midiprep Plasmid DNA kit (Qiagen Plasmid Midi Kit 100]; Qiagen, cat. no. 12145) Nuclease free water (NF-water, NEB, cat. no B1500) Blunt-end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S) Sterile LB liquid medium (Thermo Fisher, cat. no. L3522-1KG) 10× CutSmart Buffer (NEB, cat. no B6004) Alkaline phosphatase (Quick CIP or rSAP, NEB, cat. no. M0525S or M0371S) 0.7% fresh agarose gel (SeaKem LE Agarose; Lonza, cat. no. 50004) AccuGENE 5× TBE Buffer (Lonza, cat. no. 50836) Monarch DNA Gel Extraction Kit (NEB, cat. no. T1020S) 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standards #1 and #2 (Invitrogen, cat. no. Q33263) Gel box and gel electrophoresis apparatus for agarose gel Razor blade (VWR, cat. no. 55411-050) Thermal mixer (Eppendorf, cat. no. 5382000023) 1.5-ml tube (Eppendorf, cat. no. 022363301) Qubit 4 Fluorometer (Invitrogen, cat. no. Q33238) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Florez-Cardona et al. 18 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Table 5 Reaction Components for Digesting Expression Plasmid to Create Linearized Vector Component Volume Nuclease-free water a Vector a 10× CutSmart buffer 5 μl SmaI 4 μl Total volume 50 μl a Nuclease-free water, vector, and CutSmart buffer are from the midiprep kit.

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Protein expression plasmid vector of your choice containing a blunt‐end restriction site at the multi‐cloning site (see protocol introduction) Midiprep Plasmid DNA kit (Qiagen Plasmid Midi Kit 100]; Qiagen, cat. no. 12145) Nuclease free water (NF‐water, NEB, cat. no B1500) Blunt‐end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S) Sterile LB liquid medium (Thermo Fisher, cat. no. L3522‐1KG) 10× CutSmart Buffer (NEB, cat. no B6004) Alkaline phosphatase (Quick CIP or rSAP, NEB, cat. no. M0525S or M0371S) 0.7% fresh agarose gel (SeaKem LE Agarose; Lonza, cat. no. 50004) AccuGENE 5× TBE Buffer (Lonza, cat. no. 50836) Monarch DNA Gel Extraction Kit (NEB, cat. no. T1020S) 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standards #1 and #2 (Invitrogen, cat. no. Q33263) Gel box and gel electrophoresis apparatus for agarose gel Razor blade (VWR, cat. no. 55411‐050) Thermal mixer (Eppendorf, cat. no. 5382000023) 1.5‐ml tube (Eppendorf, cat. no. 022363301) Qubit 4 Fluorometer (Invitrogen, cat. no. Q33238) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) .. 125 g sucrose (Sigma‐Aldrich, cat. no. S0389‐500G) 25 ml 1 M Tris•Cl, pH 8.0 (Invitrogen, cat. no. AM9855G) Add deionized H 2 O to 500 ml final volume Store up to 6 months at 4°C.

    Spectrophotometry:

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Protein expression plasmid vector of your choice containing a blunt-end restriction site at the multi-cloning site (see protocol introduction) Midiprep Plasmid DNA kit (Qiagen Plasmid Midi Kit 100]; Qiagen, cat. no. 12145) Nuclease free water (NF-water, NEB, cat. no B1500) Blunt-end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S) Sterile LB liquid medium (Thermo Fisher, cat. no. L3522-1KG) 10× CutSmart Buffer (NEB, cat. no B6004) Alkaline phosphatase (Quick CIP or rSAP, NEB, cat. no. M0525S or M0371S) 0.7% fresh agarose gel (SeaKem LE Agarose; Lonza, cat. no. 50004) AccuGENE 5× TBE Buffer (Lonza, cat. no. 50836) Monarch DNA Gel Extraction Kit (NEB, cat. no. T1020S) 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standards #1 and #2 (Invitrogen, cat. no. Q33263) Gel box and gel electrophoresis apparatus for agarose gel Razor blade (VWR, cat. no. 55411-050) Thermal mixer (Eppendorf, cat. no. 5382000023) 1.5-ml tube (Eppendorf, cat. no. 022363301) Qubit 4 Fluorometer (Invitrogen, cat. no. Q33238) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Florez-Cardona et al. 18 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Table 5 Reaction Components for Digesting Expression Plasmid to Create Linearized Vector Component Volume Nuclease-free water a Vector a 10× CutSmart buffer 5 μl SmaI 4 μl Total volume 50 μl a Nuclease-free water, vector, and CutSmart buffer are from the midiprep kit.

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Protein expression plasmid vector of your choice containing a blunt‐end restriction site at the multi‐cloning site (see protocol introduction) Midiprep Plasmid DNA kit (Qiagen Plasmid Midi Kit 100]; Qiagen, cat. no. 12145) Nuclease free water (NF‐water, NEB, cat. no B1500) Blunt‐end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S) Sterile LB liquid medium (Thermo Fisher, cat. no. L3522‐1KG) 10× CutSmart Buffer (NEB, cat. no B6004) Alkaline phosphatase (Quick CIP or rSAP, NEB, cat. no. M0525S or M0371S) 0.7% fresh agarose gel (SeaKem LE Agarose; Lonza, cat. no. 50004) AccuGENE 5× TBE Buffer (Lonza, cat. no. 50836) Monarch DNA Gel Extraction Kit (NEB, cat. no. T1020S) 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standards #1 and #2 (Invitrogen, cat. no. Q33263) Gel box and gel electrophoresis apparatus for agarose gel Razor blade (VWR, cat. no. 55411‐050) Thermal mixer (Eppendorf, cat. no. 5382000023) 1.5‐ml tube (Eppendorf, cat. no. 022363301) Qubit 4 Fluorometer (Invitrogen, cat. no. Q33238) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) .. 125 g sucrose (Sigma‐Aldrich, cat. no. S0389‐500G) 25 ml 1 M Tris•Cl, pH 8.0 (Invitrogen, cat. no. AM9855G) Add deionized H 2 O to 500 ml final volume Store up to 6 months at 4°C.

    Solubility:

    Article Title: Efficacy of sulfonamides targeting malic enzyme in an animal model of Chagas disease
    Article Snippet: Six images per well were acquired using the Operetta system (PerkinElmer) and analyzed with Columbus software to quantify infected cells (≥3 cytoplasmic parasite spots). .. For the solubility assay, the solutions were maintained at 25 °C (Thermal Mixer, Eppendorf), and samples (200 μL) were collected at 0 and 1.5 h. For the chemical stability experiment, the compounds were evaluated under the same conditions with an additional incubation at 37 °C for a period of 24 h. The analysis was performed using an analytical method developed and validated by high-performance liquid chromatography with UV detection (HPLC-UV). ..

    Incubation:

    Article Title: Efficacy of sulfonamides targeting malic enzyme in an animal model of Chagas disease
    Article Snippet: Six images per well were acquired using the Operetta system (PerkinElmer) and analyzed with Columbus software to quantify infected cells (≥3 cytoplasmic parasite spots). .. For the solubility assay, the solutions were maintained at 25 °C (Thermal Mixer, Eppendorf), and samples (200 μL) were collected at 0 and 1.5 h. For the chemical stability experiment, the compounds were evaluated under the same conditions with an additional incubation at 37 °C for a period of 24 h. The analysis was performed using an analytical method developed and validated by high-performance liquid chromatography with UV detection (HPLC-UV). ..

    High Performance Liquid Chromatography:

    Article Title: Efficacy of sulfonamides targeting malic enzyme in an animal model of Chagas disease
    Article Snippet: Six images per well were acquired using the Operetta system (PerkinElmer) and analyzed with Columbus software to quantify infected cells (≥3 cytoplasmic parasite spots). .. For the solubility assay, the solutions were maintained at 25 °C (Thermal Mixer, Eppendorf), and samples (200 μL) were collected at 0 and 1.5 h. For the chemical stability experiment, the compounds were evaluated under the same conditions with an additional incubation at 37 °C for a period of 24 h. The analysis was performed using an analytical method developed and validated by high-performance liquid chromatography with UV detection (HPLC-UV). ..

    Software:

    Article Title: High-Complexity One-Pot Golden Gate Assembly.
    Article Snippet: NEB 10-beta Electrocompetent E. coli (New England Biolabs, cat. no. C3020K) or other suitable T7 host strain LB medium (Elbing & Brent, 2019) LB plate containing T7 phage plaques (see Basic Protocol 5) Phage dilution buffer (see recipe) PEG 8000 (Sigma-Aldrich, cat. no 89510) NaCl (Sigma-Aldrich, cat. no. 793566) Monarch HMW DNA Extraction Kit for Tissue (New England Biolabs, cat. no. T3060S) containing: Monarch HMW gDNA Tissue Lysis Buffer Proteinase K (molecular biology grade) Monarch Protein Separation Solution Monarch 2 ml Tubes Monarch DNA Capture Beads Monarch gDNA Wash Buffer Monarch Bead Retainers Monarch Collection Tubes II Monarch gDNA Elution Buffer II HPLC-grade isopropanol (Sigma-Aldrich, cat. no. 34863) Ligation Sequencing Kit (Oxford Nanopore Technologies, cat. no. SQL-LSK109) Native Barcoding Expansion (Oxford Nanopore Technologies, cat. no. EXP-NBD104 or EXP-NBD114) Adapter Mix II Expansion (Oxford Nanopore Technologies, cat. no. EXP-AMII001) NEB Blunt/TA Ligase Master Mix (New England Biolabs, cat. no. M0367) NEBNext Quick Ligation Reaction Buffer (New England Biolabs, cat. no. B6058) NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (New England Biolabs, cat. no. E7180S) Agencourt AMPure XP beads (Beckman Coulter, cat. no. A63881) MinION Flow Cell (R9.4.1) (Oxford Nanopores Technologies, cat. no. FLO-MIN106D) Flow Cell Priming Kit (Oxford Nanopore Technologies, cat. no. EXP-FLP002) Sikkema et al. 31 of 47 Current Protocols 26911299, 2023, 9, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.882 by N at Prov Indonesia, W iley O nline L ibrary on [14/01/2024]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Culture tube (VWR, cat. no. 10545-946) 37°C shaking incubator 250-ml Erlenmeyer flask (VWR, cat. no. 10536-914) 1.5-ml DNA LoBind tubes (Eppendorf, cat. no. 022431021) 50-ml centrifuge tubes (VWR, cat. no. 21008-940 or similar) Thermal mixer (e.g., Eppendorf, cat. no. 5382000023) Tube rotator (e.g., VWR, cat. no. 10136-084) Magnetic separator for 1.5-ml tubes (New England Biolabs, cat. no. S1509S) MacOS with NCBI BLAST software (https:// ftp.ncbi.nlm.nih.gov/blast/executables/blast/LATEST/ ) NOTE: As of this writing, some of the ONT kits listed (SQL-LSK109, EXP-NBD 104/114, and EXP-AMII001) are being replaced by a single kit (SQK-NBD, 114.24 Ligation Sequencing gDNA - Native Barcoding Kit 24 V14), which is used with NEB products M0367, M6630, E7546, and E6056. ..

    Ligation:

    Article Title: High-Complexity One-Pot Golden Gate Assembly.
    Article Snippet: NEB 10-beta Electrocompetent E. coli (New England Biolabs, cat. no. C3020K) or other suitable T7 host strain LB medium (Elbing & Brent, 2019) LB plate containing T7 phage plaques (see Basic Protocol 5) Phage dilution buffer (see recipe) PEG 8000 (Sigma-Aldrich, cat. no 89510) NaCl (Sigma-Aldrich, cat. no. 793566) Monarch HMW DNA Extraction Kit for Tissue (New England Biolabs, cat. no. T3060S) containing: Monarch HMW gDNA Tissue Lysis Buffer Proteinase K (molecular biology grade) Monarch Protein Separation Solution Monarch 2 ml Tubes Monarch DNA Capture Beads Monarch gDNA Wash Buffer Monarch Bead Retainers Monarch Collection Tubes II Monarch gDNA Elution Buffer II HPLC-grade isopropanol (Sigma-Aldrich, cat. no. 34863) Ligation Sequencing Kit (Oxford Nanopore Technologies, cat. no. SQL-LSK109) Native Barcoding Expansion (Oxford Nanopore Technologies, cat. no. EXP-NBD104 or EXP-NBD114) Adapter Mix II Expansion (Oxford Nanopore Technologies, cat. no. EXP-AMII001) NEB Blunt/TA Ligase Master Mix (New England Biolabs, cat. no. M0367) NEBNext Quick Ligation Reaction Buffer (New England Biolabs, cat. no. B6058) NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (New England Biolabs, cat. no. E7180S) Agencourt AMPure XP beads (Beckman Coulter, cat. no. A63881) MinION Flow Cell (R9.4.1) (Oxford Nanopores Technologies, cat. no. FLO-MIN106D) Flow Cell Priming Kit (Oxford Nanopore Technologies, cat. no. EXP-FLP002) Sikkema et al. 31 of 47 Current Protocols 26911299, 2023, 9, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.882 by N at Prov Indonesia, W iley O nline L ibrary on [14/01/2024]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Culture tube (VWR, cat. no. 10545-946) 37°C shaking incubator 250-ml Erlenmeyer flask (VWR, cat. no. 10536-914) 1.5-ml DNA LoBind tubes (Eppendorf, cat. no. 022431021) 50-ml centrifuge tubes (VWR, cat. no. 21008-940 or similar) Thermal mixer (e.g., Eppendorf, cat. no. 5382000023) Tube rotator (e.g., VWR, cat. no. 10136-084) Magnetic separator for 1.5-ml tubes (New England Biolabs, cat. no. S1509S) MacOS with NCBI BLAST software (https:// ftp.ncbi.nlm.nih.gov/blast/executables/blast/LATEST/ ) NOTE: As of this writing, some of the ONT kits listed (SQL-LSK109, EXP-NBD 104/114, and EXP-AMII001) are being replaced by a single kit (SQK-NBD, 114.24 Ligation Sequencing gDNA - Native Barcoding Kit 24 V14), which is used with NEB products M0367, M6630, E7546, and E6056. ..

    Sequencing:

    Article Title: High-Complexity One-Pot Golden Gate Assembly.
    Article Snippet: NEB 10-beta Electrocompetent E. coli (New England Biolabs, cat. no. C3020K) or other suitable T7 host strain LB medium (Elbing & Brent, 2019) LB plate containing T7 phage plaques (see Basic Protocol 5) Phage dilution buffer (see recipe) PEG 8000 (Sigma-Aldrich, cat. no 89510) NaCl (Sigma-Aldrich, cat. no. 793566) Monarch HMW DNA Extraction Kit for Tissue (New England Biolabs, cat. no. T3060S) containing: Monarch HMW gDNA Tissue Lysis Buffer Proteinase K (molecular biology grade) Monarch Protein Separation Solution Monarch 2 ml Tubes Monarch DNA Capture Beads Monarch gDNA Wash Buffer Monarch Bead Retainers Monarch Collection Tubes II Monarch gDNA Elution Buffer II HPLC-grade isopropanol (Sigma-Aldrich, cat. no. 34863) Ligation Sequencing Kit (Oxford Nanopore Technologies, cat. no. SQL-LSK109) Native Barcoding Expansion (Oxford Nanopore Technologies, cat. no. EXP-NBD104 or EXP-NBD114) Adapter Mix II Expansion (Oxford Nanopore Technologies, cat. no. EXP-AMII001) NEB Blunt/TA Ligase Master Mix (New England Biolabs, cat. no. M0367) NEBNext Quick Ligation Reaction Buffer (New England Biolabs, cat. no. B6058) NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (New England Biolabs, cat. no. E7180S) Agencourt AMPure XP beads (Beckman Coulter, cat. no. A63881) MinION Flow Cell (R9.4.1) (Oxford Nanopores Technologies, cat. no. FLO-MIN106D) Flow Cell Priming Kit (Oxford Nanopore Technologies, cat. no. EXP-FLP002) Sikkema et al. 31 of 47 Current Protocols 26911299, 2023, 9, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.882 by N at Prov Indonesia, W iley O nline L ibrary on [14/01/2024]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Culture tube (VWR, cat. no. 10545-946) 37°C shaking incubator 250-ml Erlenmeyer flask (VWR, cat. no. 10536-914) 1.5-ml DNA LoBind tubes (Eppendorf, cat. no. 022431021) 50-ml centrifuge tubes (VWR, cat. no. 21008-940 or similar) Thermal mixer (e.g., Eppendorf, cat. no. 5382000023) Tube rotator (e.g., VWR, cat. no. 10136-084) Magnetic separator for 1.5-ml tubes (New England Biolabs, cat. no. S1509S) MacOS with NCBI BLAST software (https:// ftp.ncbi.nlm.nih.gov/blast/executables/blast/LATEST/ ) NOTE: As of this writing, some of the ONT kits listed (SQL-LSK109, EXP-NBD 104/114, and EXP-AMII001) are being replaced by a single kit (SQK-NBD, 114.24 Ligation Sequencing gDNA - Native Barcoding Kit 24 V14), which is used with NEB products M0367, M6630, E7546, and E6056. ..

    other:

    Article Title: Bio-orthogonal tuning of matrix properties during 3D cell culture to induce morphological and phenotypic changes.
    Article Snippet: Described herein is a protocol for producing a synthetic extracellular matrix that can be modified in situ during three-dimensional cell culture.. The hydrogel platform is established using modular building blocks employing bio-orthogonal tetrazine (Tz) ligation with slow (norbornene, Nb) and fast (trans-cyclooctene, TCO) dienophiles.. A cell-laden gel construct is created via the slow, off-stoichiometric Tz/Nb reaction.



    Similar Products

    temperature controlled thermal mixer  (Thermo Fisher)
    90
    Thermo Fisher temperature controlled thermal mixer
    Temperature Controlled Thermal Mixer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thermal+mixer/thermal+mixer/bio_rxiv__2025__07__09__663881-318-18-19
    Average 90 stars, based on 1 article reviews
    temperature controlled thermal mixer - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    eppendorf n a thermal mixer  (Eppendorf AG)
    99
    Eppendorf AG eppendorf n a thermal mixer
    Eppendorf N A Thermal Mixer, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thermal+mixer/Eppendorf/pm42784455-98-156-156
    Average 99 stars, based on 1 article reviews
    eppendorf n a thermal mixer - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    thermal mixer  (Fisher Scientific)
    86
    Fisher Scientific thermal mixer
    Thermal Mixer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thermal+mixer/mixer+thermal/10__1016_slash_j__foodhyd__2026__112457-165-14-16
    Average 86 stars, based on 1 article reviews
    thermal mixer - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    thermal mixer  (Eppendorf AG)
    99
    Eppendorf AG thermal mixer
    Thermal Mixer, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thermal+mixer/Eppendorf/pmc12698583-166-11-13
    Average 99 stars, based on 1 article reviews
    thermal mixer - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    thermal mixer with a block  (Eppendorf AG)
    90
    Eppendorf AG thermal mixer with a block
    Thermal Mixer With A Block, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thermal+mixer/thermal+block/bio_rxiv__2025__07__17__665282-176-7-11
    Average 90 stars, based on 1 article reviews
    thermal mixer with a block - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    thermal mixer c  (Eppendorf AG)
    90
    Eppendorf AG thermal mixer c
    Thermal Mixer C, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thermal+mixer/thermal+mixer+c/bio_rxiv__2025__05__29__656739-361-16-15
    Average 90 stars, based on 1 article reviews
    thermal mixer c - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    fisherbrandtm heating thermal mixer ii  (Fisher Scientific)
    86
    Fisher Scientific fisherbrandtm heating thermal mixer ii
    Fisherbrandtm Heating Thermal Mixer Ii, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thermal+mixer/mixer+thermal/10__1016_slash_j__cej__2025__163714-82-18-23
    Average 86 stars, based on 1 article reviews
    fisherbrandtm heating thermal mixer ii - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    thermal mixer thermomix tm5  (Vorwerk Elektrowerke GmbH Co KG)
    90
    Vorwerk Elektrowerke GmbH Co KG thermal mixer thermomix tm5
    Thermal Mixer Thermomix Tm5, supplied by Vorwerk Elektrowerke GmbH Co KG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thermal+mixer/thermal+mixer+thermomix+tm5/pm40331708-139-5-9
    Average 90 stars, based on 1 article reviews
    thermal mixer thermomix tm5 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results